To better ascertain/confirm data robustness of genes predicted to be regulated by MASv5.0 algorithm, additional statistical analysis can be performed.
A software suite developed inside the Institute by Dr.G.Finocchiaro and Dr.H.Muller, by taking advantage of the replicate design (a different approach should be used for experiments performed in singlet) performs T-test and ANOVA analysis, looking for genes regulated in the same direction between the replicate.
By setting the appropriates cut-off values and using different analysis schemes, lists of genes regulated accordingly to MASv5.0 algorithm and passing a T-test with different stringency cut-off values can be obtained.
These result lists, together with fully annotated and frequently updated genes description tables, will grant you immediate attention focus on interesting regulated genes.