Overview
We use automated Dye-Terminator Sequencing (Sanger Sequencing) to provide long high quality reads from the most various DNA templates. In more than 10 years we have provided service to support hundreds of basic and translational research projects. In addition, we participate in the diagnostic activity of the CGT lab.
Service
We perform standard sequencing as well as sequencing projects. We do fragment analysis for the most different applications. We assist in primer design and in data analysis.
Innovation
We routinely revise existing protocols to increase the quality of our work. In addition, we develop new techniques according to researcher's needs.
Quality
We provide read length of 600-800 bases and universal primers free of charge. We have a rapid turn-over time (1-3 working days) and automated data delivery in .abi format. We are UNI EN ISO 9001:2008 certified.
UNI EN ISO 9001:2008 n. IT256850
Services
Sequencing
- Sequencing in single tube and 96-well format
- Primer Walking
- Re-sequencing
- Variant Analysis (SNP & Mutations)
Fragment Analysis
- Microsatellite analysis
- Cell line verification
- CrispR/Cas9 genotyping
- MLPA
Data Analysis
- Data Analysis for Re-Sequencing and Primer Walking projects
- Data Analysis for Cell line ID and CrispR/Cas9 genotyping
- Assistance in Trouble Shooting for Standard Sequencing
More technical information about the DNA Sequencing
FAQ
This is a list of our most frequently asked questions:
We provide a web-based request form. To access it your institution needs to register with Cogentech, after which your administrator will provide you with access details.
The sequencing primers should have the following characteristics:
- 18-20 bp
- G/C anchor at the 3’ and if possible at the 5’ end
- GC content of 30-70%
- Tm: 48-65 °C
- Devoid of regions that can form secondary structures or promote dimer formation
- Anneal at least 20-30 bp upstream of the region of interest
We advise to run a similarity search between your primer sequence and your target sequence to ensure that annealing occurs at a single site (Vecscreen, BLAST2seq, BLAT )
You can bring your samples and primer at Building 11, second floor any time and put them in the specific fridge. If you bring your samples after 10:00 am the reactions will be processed the day after.
Please ship your samples to: DNA Sequencing Service Cogentech Via Adamello 16 20139 Milano We accept samples from Monday to Friday. Samples that arrive after 10:00 a.m. will be processed the day after. Samples are best shipped with a cooling pad, especially for long travels and in summer.
The results are usually delivered in 1-3 working days. You will receive an email with a link from which you can download the results file.
If the sequencing reaction fails we reload the sample to exclude a technical error (thus please let us know if you are not sure a reaction might work). If the reaction fails again, we will contact you for troubleshooting.
We suggest to use any of the following freeware:
- 4 Peaks (Mac)
- Finch Tv (Mac and Windows)
- Chromas (Windows)
- Sequencing Scanner (Windows)
- Sequencer (Mac and Windows)
Staff and Contacts
Sara Volorio
Sara Volorio has obtained her degree in Biology in 1995 from the University of Milan. She then worked for 5 years at TIGEM where she discovered her passion for Sequencing. She moved to IFOM in 2000 where she became a pilar of the Sequencing facility.
Stefano Fortuzzi
Stefano Fortuzzi obtained his degree in Agricultural Biotecnology at the University of Bologna in 2005. During the following years he joined the Diagnostic team of Cogentech focused on developing automated processes. Furthermore he started a collaboration with the Sequencing facility in 2014.
Domenico Sardella
Domenico Sardella visited the University of Urbino and obtained his degree as Biomedical Laboratory Technician in 2003. He has worked at the “Istituto di Tumori” of Milano and at the “European Insitute of Oncology” before joining the Sequencing team in 2012.
Contacts
- Phone
+39 02 574303210
+39 02 574303207 (lab) - Email
sequencing-service [@] cogentech.it
