We perform gene expression profiling on total RNA, miRNA, mRNA from cells or tissues from human, mouse, rat and other species.
We perform genomic analysis on human samples for molecular karyotyping, copy number variation or genotyping, using high density Affymetrix® microarrays.
This is a list of our most frequently asked questions regarding the work-flow of the facility:
You can write to us at genomicunit[@]cogentech.it for any question or information.
Generally we ask you contact us (genomicunit[@]cogentech.it) to discuss your needs and define the experimental outline. Based on this, our administration will send you a commercial offer. Upon acceptance of the offer and receipt of the request form we can start processing your samples.
Our facility offers a full service, which includes arrays and reagents. If however you prefer to buy your own arrays or reagents, feel free to do so.
We work with all array types available from Affymetrix. If you already know what you need you can go directly to the Affymetrix website and choose your preferred array. If you are unsure which is the best array type please contact us and we will guide you through the selection.
Yes, we can offer you the data analysis for an additional cost.
No, we accept only RNA/DNA.
For Gene Expression Profiling: at least 500 ng of total RNA resuspended in 10-30 μl of RNase-free water, we highly recommend Dnase treatment to avoid contamination with genomic DNA.
For miRNA Analysis: at least 1μg in 10 ul of RNase-free water.
For Whole-Genome Copy Number Analysis: at least 500 ng of genomic DNA resuspended in 10-15 μl of nuclease-free water.
Yes, we have special protocols for little amount of starting RNA. Please contact us to discuss how to best meet your experimental needs. genomicunit[@]cogentech.it
We evalutate RNA quality on an Agilent Bioanalyzer.
All samples must be shipped at a controlled temperature (RNA on dry ice, DNA at +4 °C). Please plan your shipment in a way to avoid delivery after 5:00 pm on Fridays or on weekends. Shipments will not be collected at these times. It is your duty to avoid that during transport or in storage samples remain at an uncontrolled temperature.
Our shipping address is:
Via Adamello 16
Leftover samples will be stored for 4 months, after which they will be discarded. We will be happy to send you back your remaining samples before that deadline, but shipping costs will be at your charge.
You will receive an email with a link from which you can download the results files. Raw data will be available as .CEL files. In case you asked for data analysis, these results will be delivered in txt or jpg format.
Please contact us at genomicunit[@]cogentech.it.